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human active gst yes1  (MedChemExpress)


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    Structured Review

    MedChemExpress human active gst yes1
    mtiRL enhances the binding between <t>Yes1</t> and ANXA2. A,B) Endogenous Co‐immunoprecipitation (co‐IP) assays revealed that Yes1 bound to ANXA2. C,D) Exogenous co‐IP assays confirmed that GFP‐Yes1 interacted with Flag‐ ANXA2. E) Colocalization of Yes1 and ANXA2 was confirmed by immunofluorescence staining in Cd‐SV‐HUC‐1 cells. F–I) WB results showed that overexpression of Yes1 upregulated the level of p‐ANXA2‐Y24, whereas it did not affect the expression of total ANXA2 protein. J–M) Co‐IP experiments in Cd‐SV‐HUC‐1 cells revealed that the interaction between endogenous Yes1 and ANXA2 was significantly diminished by knockdown of mtiRL. N) In vitro kinase assay indicated that <t>active</t> <t>GST‐Yes1</t> could phosphorylate ANXA2 at Tyr24 site and mtiRL enhance the phosphorylation of Yes1. * p < 0.05, ** p < 0.01.
    Human Active Gst Yes1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+active+gst+yes1/YES1%2C+Human/pmc11336930-243-14-19
    Average 92 stars, based on 7 article reviews
    human active gst yes1 - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "A Novel tsRNA, m 7 G‐3′ tiRNA Lys TTT , Promotes Bladder Cancer Malignancy Via Regulating ANXA2 Phosphorylation"

    Article Title: A Novel tsRNA, m 7 G‐3′ tiRNA Lys TTT , Promotes Bladder Cancer Malignancy Via Regulating ANXA2 Phosphorylation

    Journal: Advanced Science

    doi: 10.1002/advs.202400115

    mtiRL enhances the binding between Yes1 and ANXA2. A,B) Endogenous Co‐immunoprecipitation (co‐IP) assays revealed that Yes1 bound to ANXA2. C,D) Exogenous co‐IP assays confirmed that GFP‐Yes1 interacted with Flag‐ ANXA2. E) Colocalization of Yes1 and ANXA2 was confirmed by immunofluorescence staining in Cd‐SV‐HUC‐1 cells. F–I) WB results showed that overexpression of Yes1 upregulated the level of p‐ANXA2‐Y24, whereas it did not affect the expression of total ANXA2 protein. J–M) Co‐IP experiments in Cd‐SV‐HUC‐1 cells revealed that the interaction between endogenous Yes1 and ANXA2 was significantly diminished by knockdown of mtiRL. N) In vitro kinase assay indicated that active GST‐Yes1 could phosphorylate ANXA2 at Tyr24 site and mtiRL enhance the phosphorylation of Yes1. * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: mtiRL enhances the binding between Yes1 and ANXA2. A,B) Endogenous Co‐immunoprecipitation (co‐IP) assays revealed that Yes1 bound to ANXA2. C,D) Exogenous co‐IP assays confirmed that GFP‐Yes1 interacted with Flag‐ ANXA2. E) Colocalization of Yes1 and ANXA2 was confirmed by immunofluorescence staining in Cd‐SV‐HUC‐1 cells. F–I) WB results showed that overexpression of Yes1 upregulated the level of p‐ANXA2‐Y24, whereas it did not affect the expression of total ANXA2 protein. J–M) Co‐IP experiments in Cd‐SV‐HUC‐1 cells revealed that the interaction between endogenous Yes1 and ANXA2 was significantly diminished by knockdown of mtiRL. N) In vitro kinase assay indicated that active GST‐Yes1 could phosphorylate ANXA2 at Tyr24 site and mtiRL enhance the phosphorylation of Yes1. * p < 0.05, ** p < 0.01.

    Techniques Used: Binding Assay, Immunoprecipitation, Co-Immunoprecipitation Assay, Immunofluorescence, Staining, Over Expression, Expressing, Knockdown, In Vitro, Kinase Assay, Phospho-proteomics

    Related Articles

    Recombinant:

    Article Title: A Novel tsRNA, m 7 G‐3′ tiRNA Lys TTT , Promotes Bladder Cancer Malignancy Via Regulating ANXA2 Phosphorylation
    Article Snippet: .. [ ] Recombinant human His‐ANXA2 (100 ng; Jingxin Bio, #GXP87073) was incubated with recombinant human active GST‐Yes1 (100 ng; MCE, # HY‐P73486) and mtiRL (1 μg) at 30 °C for 30 min in reaction buffer (20 mM HEPES (pH 7.6), 20 mM MgCl 2 , 0.2 mM ATP, 2 mM DTT, 20 mM β‐glycerophosphate, 0.1 mM sodium orthovanadate). ..

    Incubation:

    Article Title: A Novel tsRNA, m 7 G‐3′ tiRNA Lys TTT , Promotes Bladder Cancer Malignancy Via Regulating ANXA2 Phosphorylation
    Article Snippet: .. [ ] Recombinant human His‐ANXA2 (100 ng; Jingxin Bio, #GXP87073) was incubated with recombinant human active GST‐Yes1 (100 ng; MCE, # HY‐P73486) and mtiRL (1 μg) at 30 °C for 30 min in reaction buffer (20 mM HEPES (pH 7.6), 20 mM MgCl 2 , 0.2 mM ATP, 2 mM DTT, 20 mM β‐glycerophosphate, 0.1 mM sodium orthovanadate). ..



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    human active gst yes1  (MedChemExpress)
    92
    MedChemExpress human active gst yes1
    mtiRL enhances the binding between <t>Yes1</t> and ANXA2. A,B) Endogenous Co‐immunoprecipitation (co‐IP) assays revealed that Yes1 bound to ANXA2. C,D) Exogenous co‐IP assays confirmed that GFP‐Yes1 interacted with Flag‐ ANXA2. E) Colocalization of Yes1 and ANXA2 was confirmed by immunofluorescence staining in Cd‐SV‐HUC‐1 cells. F–I) WB results showed that overexpression of Yes1 upregulated the level of p‐ANXA2‐Y24, whereas it did not affect the expression of total ANXA2 protein. J–M) Co‐IP experiments in Cd‐SV‐HUC‐1 cells revealed that the interaction between endogenous Yes1 and ANXA2 was significantly diminished by knockdown of mtiRL. N) In vitro kinase assay indicated that <t>active</t> <t>GST‐Yes1</t> could phosphorylate ANXA2 at Tyr24 site and mtiRL enhance the phosphorylation of Yes1. * p < 0.05, ** p < 0.01.
    Human Active Gst Yes1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+active+gst+yes1/YES1%2C+Human/pmc11336930-243-14-19
    Average 92 stars, based on 1 article reviews
    human active gst yes1 - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    mtiRL enhances the binding between Yes1 and ANXA2. A,B) Endogenous Co‐immunoprecipitation (co‐IP) assays revealed that Yes1 bound to ANXA2. C,D) Exogenous co‐IP assays confirmed that GFP‐Yes1 interacted with Flag‐ ANXA2. E) Colocalization of Yes1 and ANXA2 was confirmed by immunofluorescence staining in Cd‐SV‐HUC‐1 cells. F–I) WB results showed that overexpression of Yes1 upregulated the level of p‐ANXA2‐Y24, whereas it did not affect the expression of total ANXA2 protein. J–M) Co‐IP experiments in Cd‐SV‐HUC‐1 cells revealed that the interaction between endogenous Yes1 and ANXA2 was significantly diminished by knockdown of mtiRL. N) In vitro kinase assay indicated that active GST‐Yes1 could phosphorylate ANXA2 at Tyr24 site and mtiRL enhance the phosphorylation of Yes1. * p < 0.05, ** p < 0.01.

    Journal: Advanced Science

    Article Title: A Novel tsRNA, m 7 G‐3′ tiRNA Lys TTT , Promotes Bladder Cancer Malignancy Via Regulating ANXA2 Phosphorylation

    doi: 10.1002/advs.202400115

    Figure Lengend Snippet: mtiRL enhances the binding between Yes1 and ANXA2. A,B) Endogenous Co‐immunoprecipitation (co‐IP) assays revealed that Yes1 bound to ANXA2. C,D) Exogenous co‐IP assays confirmed that GFP‐Yes1 interacted with Flag‐ ANXA2. E) Colocalization of Yes1 and ANXA2 was confirmed by immunofluorescence staining in Cd‐SV‐HUC‐1 cells. F–I) WB results showed that overexpression of Yes1 upregulated the level of p‐ANXA2‐Y24, whereas it did not affect the expression of total ANXA2 protein. J–M) Co‐IP experiments in Cd‐SV‐HUC‐1 cells revealed that the interaction between endogenous Yes1 and ANXA2 was significantly diminished by knockdown of mtiRL. N) In vitro kinase assay indicated that active GST‐Yes1 could phosphorylate ANXA2 at Tyr24 site and mtiRL enhance the phosphorylation of Yes1. * p < 0.05, ** p < 0.01.

    Article Snippet: [ ] Recombinant human His‐ANXA2 (100 ng; Jingxin Bio, #GXP87073) was incubated with recombinant human active GST‐Yes1 (100 ng; MCE, # HY‐P73486) and mtiRL (1 μg) at 30 °C for 30 min in reaction buffer (20 mM HEPES (pH 7.6), 20 mM MgCl 2 , 0.2 mM ATP, 2 mM DTT, 20 mM β‐glycerophosphate, 0.1 mM sodium orthovanadate).

    Techniques: Binding Assay, Immunoprecipitation, Co-Immunoprecipitation Assay, Immunofluorescence, Staining, Over Expression, Expressing, Knockdown, In Vitro, Kinase Assay, Phospho-proteomics